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Fig. 3. Interphase FISH with band-specific probes for chromosome 8 and 9 on human fibroblasts. (A) G-banded chromosomes 8 and 9, showing the position and size of the bands covered by the applied chromosome band-specific probes 8p11.2 (green) and 9q12-13 (red). (B) Example of confluent human fibroblasts after hybridization. Criteria for manual analysis were made according to the number of hybridization signals in the nucleus. Discrimination was made between cells with: two separate hybridization signals for each probe (left) and nuclei in which signals of homologous chromosomes were so close together that only one single, usually larger and brighter, hybridization signal (middle) or two touching signals (right) could be observed.
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