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Fig. 5. Position of chromosome bands 8p11.2 and 9q12-13 within confluent fibroblasts. (A) The position of hybridization signals observed in 200 nuclei from control and MMC-treated confluent human fibroblasts are plotted in one quadrant of the nucleus. The outer curve represents the contours of an average sized nucleus. The inner curve corresponds to one quadrant of a circle with a radius equal to the median of the radial distances of the hybridization signals. Therefore, 50% of hybridization signals are located inside this circle. (B) The inter-homologue distance distribution of chromosome bands 8p11.2 and 9q12-13 was determined by analyzing 200 nuclei from control and MMC-treated confluent human fibroblasts. Cells with ascending inter-homologue distances are plotted against the inter-homologue distances.





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