QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 2. HU- and IR-induced modifications of Cdc25^Stg and Cdc2 in control S2 cells but not in Grp/DChk1-depleted S2 cells. Wild-type embryos at 0-2 hours of age (lane 1) expressed the active phosphorylated isoform (form 1) and the unphosphorylated neutral isoform (form 2) of Cdc2. Wild-type embryos at 2-4 hours of age (lane 2) showed accumulation of inhibitory phosphorylated isoforms (forms 3 and 4) of Cdc2. In early embryos (lane 1) Cdc25^Stg was expressed, whereas Cdc25^Stg protein was almost absent from in older embryos (lane 2). In S2 cells (lanes 3 and 5), Cdc2 isoforms 2-4 were detected and Cdc25^Stg was present. In S2 cells treated with 10 mM HU during 15 hours (lane 4) the Cdc2 isoforms 3 and 4 accumulated, Cdc2 isoform 2 was almost absent and Cdc25^Stg levels decreased. S2 cells treated with 150 Gy IR followed by 2 hours of recovery (lane 6) showed accumulation of Cdc2 isoform 4 and presence of Cdc2 isoforms 2 and 3. Cdc25^Stg levels decreased moderately in response to IR. S2 cells depleted for Grp/DChk1 by RNAi (lanes 7 and 9) expressed Cdc25^Stg and all Cdc2 isoforms (forms 2-4). Grp/DChk1-depleted cells treated with 10 mM HU (lane 8) or treated with 150 Gy IR followed by 2 hours recovery (lane 10) did not show modifications of Cdc25^Stg and Cdc2. Equal numbers (n=20) of Drosophila embryos were loaded in lanes 1 and 2. For lanes 3-10, a background band (Bg) recognized by the Cdc25^Stg antibody was used as a loading control.

Return to article