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Fig. 6. Analysis of mitotic chromosomes from wild-type S2 cells and Grp/DChk1-depleted S2 cells in response to HU or IR. S2 cells were grown on cover slips and mitotic chromosomes were labelled with the anti-PH3 antibody. Images were made by confocal microscopy. (A-C) Mitotic chromosomes of Grp/DChk1-depleted S2 cells under control conditions in prophase (A), metaphase (B) and anaphase (C). (D,E) Fragmentation of mitotic chromosomes in Grp/DChk1-depleted cells treated with 10 mM HU (15 hours) (D) or IR (150 Gy, 2 hours recovery) (E). Scale bar, 1 µm.





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