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Fig. 2. End4/Sla2 talin-like domain is required for actin organization at new growth zones. (A) Schematic organization of End4/Sla2 mutant proteins generated in these study and percentage of cells with monopolar growth in each strain. (B) Wild-type (left) and sla2 ${Delta}$ talin (right) cells were stained with calcofluor (upper panel) and for actin (lower panel). (C) Western blot analysis of total extracts from wild-type, Sla2-GFP and Sla2 ${Delta}$ talin-GFP strains with ${alpha}$ -GFP (top) and ${alpha}$ -tubulin (bottom) antibody. (D) Patterns of growth of sla2 ${Delta}$ talin, tea1 ${Delta}$ and sla2 ${Delta}$ talin tea1 ${Delta}$ cells after division.

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