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Fig. 4. End4/Sla2 localization in vivo. (A) Sla2-GFP accumulation (green) at cell ends during interphase (top) and in a medial ring over the nucleus (middle), which persists until the end of mitosis (bottom). The nucleus is stained with DAPI (blue). (B) Central accumulation of Sla2-GFP is unaffected in tea1{Delta} cells (arrow). Sla2-GFP is enriched only at the growing end in tea1{Delta} and (C) in tea1{Delta}(200) cells. (D) Accumulation of Sla2-GFP (bottom) and actin (top) at the growing end in cdc10-129 cells. (E) Staining of Sla2-GFP-expressing cells with {alpha}-actin and (F) {alpha}-Tea1 antibody. Bar, 5 µm.





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