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Fig. 6. Sla2-GFP localization depends on vesicular transport. (A) Sla2-GFP cells were treated with (a) ethanol and DMSO, (b) 40 µM latA (10 minutes at 25°C), (c) 100 µg/ml of brefeldin A (BfA) (10 minutes at 25°C), or (d) 100 µg/ml BfA and 40 µM latA (10 minutes at 25°C). BfA treatment delocalizes Sla2-GFP in 5 minutes. Sla2-GFP de-localization is abolished by co-treatment with latA. (B) Localization of Sla2{Delta}talin-GFP (a) and calcofluor staining (b). Sla2{Delta}talin-GFP cells treated with BfA at 25°C for 10 minutes (c). (C) Staining of Sla2{Delta}talin-GFP-expressing cells with Rhodamine phalloidin. (D) Sla2-GFP local movement is abolished by treatment with 40 µM latA. Arrowheads point to the same GFP dot in different frames of a time-lapse recording (see Movie 1 in supplementary material; +latA frames are from Movie 2). Bar, 5 µm.





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