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Fig. 5. NIH-3T3 cells were transfected with PAK4 and GEF-H1S alleles and examined by fluorescence imaging of EGFP for GEF-H1 alleles, PAK4 constructs were identified by immunostaining using PAK4 antiserum followed by anti-rabbit fluorescent rhodamine conjugate for PAK4 and fluorescent coumarin-phallicidin to label actin. (A-C) GEF-H1S co-transfected with PAK4 alleles WT,S474E and K350,351A. (D-F) GEF-H1S-S810A, GEF-H1S-S67A and GEF-H1S-Q312M,R313G together with PAK4-S474E. Arrow a highlights filopodia; arrow b highlights lamellipodia induction for cells co-expressing PAK4-S474E and GEF-H1S or GEF-H1S-S67A; arrow c marks cells with stress fibers induction. Bar, 10 µm.





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