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Fig. 1. Bcl6–/– macrophages have a morphology distinct from that of Bcl6+/+ BMM. BMM were plated on tissue culture plastic (A,B) and fibronectin-coated coverslips (C,D) and grown to subconfluence in the presence of CSF1 before fixation. Phase-contrast (A,B) and scanning EM (C,D) images of Bcl6+/+ (A,C) and Bcl6–/– BMM (B,D). (E) Polarization indices of Bcl6+/+ (+/+) and Bcl6–/– (–/–) BMM (Polarization index=ratio of maximum cell length to maximum cell width, n>30; error bars represent s.e.m.; *P<0.001 significantly different from the index in Bcl6+/+ BMM cells). (F) Numbers of pseudopodia per cell in Bcl6+/+ and Bcl6–/– BMM (for maximum cell length/maximum cell width ratio ≤1.5, pseudopodia count=1; n=100). Bar, 20 µm (A,B); 10 µm (C,D).





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