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Fig. 4. Spreading is delayed in Bcl6+/+ macrophages and is associated with altered focal complex remodeling. Bcl6+/+ and Bcl6–/– BMM were plated on fibronectin-coated coverslips for the indicated times, fixed and examined by confocal microscopy. (A) BMM fixed 1, 2, 4, 6, 8 and 24 hours after plating and examined by interference reflection microscopy. (B) BMM fixed 3 hours after plating and examined by IRM and immunofluorescence staining for phosphoY118 paxillin. (C) Analysis of mean cell footprint areas at 15 and 30 minutes, 1, 2, 4, 6, 8 and 24 hours (Bcl6+/+, black, Bcl6–/–, gray; mean±s.e.m. of cells in six fields). Bar, 10 µm.





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