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Fig. 8. Expression of SCAMP2 ${Delta}$ 184-208 shows scattered vesicular appearance with NHE7 and full-length SCAMP. (A) CHO cells were transfected with myc-tagged ${Delta}$ 184-208 or full-length SCAMP2 and 1D4-tagged NHE7 (NHE7) and viewed by immunofluorescence microscopy. SCAMP2/ ${Delta}$ 184-208 had a more scattered vesicular distribution than full-length wild-type SCAMP2. Co-transfected NHE7 was redistributed to scattered vesicular structures mostly in the same compartment as SCAMP2/ ${Delta}$ 184-208. (B) CHO cells were simultaneously transfected with myc-tagged SCAMP2 deletion mutants and full-length HA-tagged SCAMP2, and their intracellular localization was visualized by immunofluorescence confocal microscopy. Expression of SCAMP2/ ${Delta}$ 184-208, but not other mutants, redistributed full-length SCAMP2 to the same scattered vesicular structure. (C) CHO cells were transiently co-transfected with full-length HA-tagged SCAMP2 and myc-tagged SCAMP2/ ${Delta}$ 184-208 or SCAMP2/ ${Delta}$ 151-174. Cell lysates (Lys) were immunoprecipitated with a mouse anti-HA antibody (HA) or pre-immune serum (con) and co-precipitated SCAMP2/ ${Delta}$ 184-208 and SCAMP2/ ${Delta}$ 151-174 were detected in a western blot by rabbit anti-myc antibody. 5% of total cell lysate was loaded. Bar, 10 µm.

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