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Fig. 9. SCAMP2/ ${Delta}$ 184-208 colocalizes with the transferrin receptor at the peripheral region of cells. PC12 cells were transiently transfected with myc-tagged SCAMP2/ ${Delta}$ 184-208 and its intracellular localization was analyzed by double-labelled immunofluorescence confocal microscopy with different organellar markers. (A) SCAMP2/ ${Delta}$ 184-208 (green) colocalized with transferrin receptor (TfR, red) at the peripheral region of cells. (B) Neither syntaxin 6 (Stx6, red) nor GM130 (red) showed significant association with SCAMP2/ ${Delta}$ 184-208 (green). Note that the expression of SCAMP2/ ${Delta}$ 184-208 did not appreciably alter localization of these markers (see Fig. 4). Bar, 10 µm.

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