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Fig. 1. Chemotaxis of gbpC and gbpD mutants. (A) Cell tracks of representative wild-type (WT) and mutant Dictyostelium cells in a spatial gradient of cAMP. Cells were starved until the ripple stage was reached. Subsequently, they were placed in a chemotaxis chamber filled with phosphate buffer at one end and phosphate buffer with cAMP at the other end. Images were taken every 10 seconds for a period of 5 minutes and the cell outlines of subsequent images were placed on top of each other to obtain the final picture. (B) Cell tracks and separate images of a wild-type and a gbpD cell that were placed in a chemotaxis chamber. The tracks are composed of stacked images that were taken every 4 seconds. Every third frame is also shown as a separate image.