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Fig. 3. Morphology of Dictyostelium cells overexpressing GbpD protein. Confocal images of starved gbpD– and gbpD–/GbpDOE cells expressing the F-actin binding domain of LimE fused to GFP. The DIC image and the uppermost fluorescent image were taken just above the glass surface. The second fluorescent image was taken ~2 µm above the glass surface. The thickness of the images corresponds to ~1 µm. The cross section image was obtained by making use of the projection option of the Zeiss LSM Image browser 5 program. White arrowheads indicate the height of the upper two fluorescent images. Bar, 10 µm.





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