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Fig. 5. cAMP-induced myosin II translocation. Wild-type cells and mutant cells expressing myosin II-GFP were starved and stimulated with cAMP. (A) Confocal fluorescent images were taken every 5 seconds and are shown at the indicated time points after cAMP stimulation. The gbpC^– cells contained unusual amounts of vacuoles in this particular experiment. (B) Quantification of the data using the QuimP software developed by T. Bretschneider (Dormann et al., 2002). Fluorescence at the membrane and in the cytosol was calculated and the ratio of the fluorescence at the membrane divided by the average cytosolic fluorescence was plotted against time. In gbpD^– (x) and wild-type cells (x), myosin translocates to the cortex after an initial depletion. In gbpC^– cells (x), this myosin translocation to the cortex is absent and depletion of the fluorescent signal from the cortex is prolonged and enhanced. The displayed data are averaged values from 13 wild-type cells, nine gbpC^– cells and seven gbpD^– cells from two or three experiments.

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