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Fig. 7. Zinc increases in vitro vesicular glutamate uptake. (A) SLMV-enriched fractions were prepared from untransfected (wt) and Vglut1-transfected (Vgl) cells. Vesicle-specific [3H]-glutamate uptake was determined in either the absence or the presence of the ionophore valinomycin (20 µM). Vesicular glutamate uptake is detected only in SLMV fractions from Vglut1-transfected cells (n=2). (B) [3H]-Glutamate uptake was performed in either the absence or the presence of increasing concentrations of ionic zinc (ZnSO4). Equal amounts of Vgl and VglZn6 SLMV protein were used in all assays. The protein content per assay was confirmed by immunoblot using antibodies against synaptic-vesicle markers.





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