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Fig. 7. Kir/Gem downregulates Ca2+ channel activity. (A) PC12 cells were cotransfected with a GFP plasmid and cDNAs for wt or mutated Kir/Gem, either with or without 14-3-3 or 14-3-3 K49E. GFP-positive cells were selected for electrophysiology and the average of the maximal current detected at +20 mV for endogenous Ca2+ channels was measured. Between 9 and 16 independent experiments were carried out for each condition. (B) An example of the I-V relationship of Ca2+ channels in PC-12. A, Ampere; F, Faraday. Cells transfected only with the GFP cDNA served as a control.





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