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Fig. 1. Kir2.1 and Kir2.4 show different levels of surface expression. (A) Representative confocal images of OK cells expressing GFPKir2.1 and GFPKir2.4. Note that only GFPKir2.1 shows distinct plasma membrane fluorescence (arrow). (B) Surface expression of extracellularly HA-tagged GFPKir2.1 and GFPKir2.4 detected by {alpha}-HA immunocytochemistry without membrane permeabilization (TX, Triton X-100; see Materials and Methods). (C) Cartoon depicting the membrane topology of Kir channels. The cytoplasmic N- and C-terminal domains contain trafficking signals for efficient ER and Golgi export, respectively (Ma et al., 2001; Stockklausner et al., 2001; Stockklausner and Klöcker, 2003). (D) Sequence alignment of the known trafficking signals in Kir2.1 and Kir2.4.





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