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Fig. 6. The identified Golgi-to-plasma membrane trafficking motif is recognized by the neuronal sorting machinery. (A) Representative confocal images of hippocampal neurons expressing extracellularly HA-tagged GFPKir2.4 and GFPKir2.4-int(2.1), respectively. Surface expression of channel constructs was determined by {alpha}-HA immunocytochemistry without membrane permeabilization (TX, Triton X-100). The clustered distribution of GFPKir2.4-int(2.1) is artificially induced by the in vivo surface-labeling procedure. (B) Quantification of surface expression (n=10 neurons). Cy-3 fluorescence intensity values of neuronal surface stainings were corrected for background and related to total protein expression measured by GFP fluorescence. Data are given as relative intensity values per µm2. *Statistically significant when compared with respective control (P<0.01; unpaired Student's t-test; see Materials and Methods).





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