QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 1. GLR-1 tail sequences are important for heteromeric AMPA receptor synaptic localization. (Top) The sequences of GLR-1 included in the GLR-1::GFP and GLR-1(tailless)::GFP transgenes. The arrowhead indicates the position of the fluorescent tag (between a.a. 947-948 for full length, and after the final transmembrane domain for the tailless form) used to monitor the protein. (A,C,E,F) GLR-1(tailless)::GFP and (B,D) GLR-1::GFP expressed in different genetic backgrounds. Pictures are of the ventral cord 100 µm anterior of the vulva. (A,B) Wild type, (C,D) glr-1 and glr-2 double deletion, (E) glr-1 deletion, and (F) glr-2 deletion. In (E) and (F), endogenous full-length GLR-2 and GLR-1, respectively, can bring the tailless GLR-1::GFP to the synapse. By contrast, there is no synaptic localization along the ventral nerve cord for (C) GLR-1(tailless)::GFP alone without the endogenous GLR-1 and GLR-2. Bar, is 5 µm. (G) The mean number of clusters per 10 µm of ventral cord length, and (H) the mean cluster size is plotted for the given genotype. `GLR-1' indicates the presence of the glr-1::gfp transgene, whereas `GLR-1T' indicates the presence of the glr-1(tailless)::gfp transgene. Error bars are s.e.m. for all graphs. **P<0.01 compared with GLR-1::GFP in wild type by ANOVA/Dunnett's multiple comparison test. n=20 animals for each genotype.

Return to article