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Fig. 7. Phosphorylation of protein kinases following treatment with light and increasing concentrations of Pd-Bchl-Ser. Cells were preincubated with increasing concentrations of Pd-Bchl-Ser, illuminated for 10 minutes and reincubated for 45 minutes. Cell lysates were prepared and proteins were separated on 10% SDS-PAGE, blotted and immunodetected with anti-phosphorylated p38, ERK, JNK or Akt. Phosphorylation is presented as a percentage of the band density displaying the highest value on the respective blot.