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Fig. 6. K8–/– colon histology following treatment with broad-spectrum oral antibiotics. (A) Hematoxylin and Eosin staining of proximal colons from K8–/– mice that were given normal drinking water for 8 weeks (a) or water containing vancomycin and imipenem at 50 mg/kg body weight (b). Significant inflammation (arrows) is present in the non-treated K8–/– colon. L, lumen; M, muscle layer. Scale bar: 10 µm, (B) Summary of the colon inflammation histology score (as described in Materials and Methods) is shown for the non-treated (–Antibiotics) and treated (+Antibiotics) groups. Data is presented as mean±s.e.m. (n=4). *P<0.005 when comparing untreated K8–/– with K8+/+, **P<0.01 when comparing untreated with treated K8–/–.

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