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Fig. 7. Effect of oral antibiotic treatment on AE1/2 ion transporter localization. Proximal colon from non-antibiotic-treated K8+/+ (a), K8–/– (b), and antibiotic-treated K8–/– (c) mice were fixed in formalin, paraffin embedded and sectioned, then double-stained with anti-AE1/2 (red) and nuclear dye (blue). Note the reversal and normalization of the brighter and supranuclear AE1/2 staining in K8–/– colon (b, arrows) following antibiotic treatment (c), to resemble the staining of non-antibiotic treated K8+/+ colon (a). Scale bar: 50 µm.





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