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Fig. 3. SDF-1 and PTN stimulate proliferation of primary keratinocytes in vitro. (A) Heterologous feeder-layer co-cultures grown in the presence or absence of recombinant human SDF-1 (50 ng/ml) or PTN (25 ng/ml) were analysed by phase contrast microscopy (left column) and immunofluorescence (right columns). Individual keratinocyte islands are outlined with dashed lines in the phase contrast images. Differentiation and proliferation of primary keratinocytes was visualized by staining for keratin 10 (early differentiation), involucrin and MIB1 (proliferation). (B) Quantification of the mitogenic effect of PTN and SDF-1 on primary keratinocytes. MIB1-positive cells of at least 10 colonies were counted and plotted with the total number of cells per keratinocyte colony (shaded bars).





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