|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 5. Regulation of PTN and SDF-1 in wounds and co-cultured fibroblasts. (A) Real-time PCR and (B) semi-quantitative RT-PCR was performed on cDNA from full-thickness excisional mouse dorsal skin wounds prepared at the indicated times after injury. (A) Changes in mRNA levels during the wound healing process are plotted relative to the expression in unwounded skin (0 hours, set as 1). Average values of three independent measurements are shown. (B) Expression analysis on RNA from an independent wounding experiment. Essentially identical results were obtained in a third independent RNA analysis of expression kinetics during wound healing (data not shown). Gro1 (A,B) and Kgf (B), both known to be induced by wounding, were included as positive controls. (C) Expression of Ptn, Sdf-1 and Cxcr4 in fibroblast monocultures or co-cultures with primary human normal epidermal keratinocytes (NEKs). Ptn and Cxcr4 were upregulated, whereas Sdf-1 transcript levels decreased in the presence of co-cultured keratinocytes.
|
