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Fig. 3. U18666A induces MLB expression despite leupeptin treatment. Mv1Lu (A,C) and Mgat5-transfected M9 (B,D) cells were incubated with leupeptin (2 µg/ml) for 96 hours and U18666A was added to selected cultures (C,D) for the final 24 hours before processing for electron microscopy. In the absence of U18666A, leupeptin induces the accumulation of dense autophagic vacuoles however U18666A still induces MLB production in leupeptin-treated cells. Bar, 2.5 µm.





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