QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 4. U18666A treatment induces accumulation of swollen LAMP-2-positive vacuoles in the presence of the autophagy inhibitor 3-MA. Untransfected Mv1Lu (A-D,I-L) and Mgat5-transfected M9 (E-H,M-P) cells were grown in medium supplemented with 10 mM 3-MA for 3 days and selected cultures were incubated with U18666A for the final 24 hours (I-P). The cells were fixed and then triple labelled with anti-LAMP-2 (A,E,I,M), Nile Red (B,F,J,N) and filipin (C,G,K,O). Merged images (D,H,L,P) show LAMP-2 in green, Nile Red in red and filipin in blue. Large phospholipid-rich, LAMP-2-positive vacuoles are not observed following 3-MA treatment but U18666A is still able to induce the formation of swollen, filipin-positive lysosomal vacuoles. Bar, 5 µm.

Return to article