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Fig. 9. 3-MA treatment does not affect fluid-phase endocytosis to lysosomes. M9 cells were grown in the presence of 3-MA for 3 days and then incubated with FITC-dextran for 30 minutes (A-C) or 4 hours (D-F) prior to labelling for LAMP-2 (A,D). FITC-dextran labelling (B,E) is shown in green and LAMP-2 in red in the merged images (C,F). FITC-dextran does not accumulate in lysosomal structures at 30 minutes but reaches them after 4 hours of endocytosis. Bar, 5 µm.





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