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Fig. 2. Concentration curve of CaM kinase inhibitors effect upon human sperm motility and viability. Immediately after selection using glass wool columns, motile sperm were resuspended in medium not supplemented with CaCl2, and in the presence or absence of the CaM kinase inhibitors KN62 (
) or KN93 (
); or their inactive analogues KN04 (
) and KN92 (
) at the following final concentrations: 1, 3, 10, 30, 60, 100 µM. After 30 minutes, aliquots were supplemented with CaCl2 (final concentration: 2.5 mM) and cells were incubated for 18 hours in capacitating conditions. Aliquots incubated in medium not supplemented with CaCl2 (– Ca2+, 
), in the absence of the inhibitors (+ Ca2+, 
), or in the presence of 0.5% DMSO (DMSO, ) served as controls. The percentages of progressively motile (A) and live (B) cells were determined. The results are expressed as mean±s.e.m., n=6. *P<0.01 when compared to sperm motility under all other conditions.
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