spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 3. Time curve of the effect of CaM kinase inhibitor (KN62) and its inactive analogue (KN04) upon human sperm motility and viability. Immediately after selection with glass wool columns, motile sperm were resuspended in medium not supplemented with CaCl2, and 60 µM KN62 ({bullet}) or KN04 ({diamondsuit}) were added. After 30 minutes, aliquots were supplemented with CaCl2 (final concentration: 2.5 mM) and cells were incubated for up to 18 hours in capacitating conditions. Aliquots incubated in medium not supplemented with CaCl2 (–Ca2+, {square}) and in the absence of the inhibitors (+Ca2+, {triangledown}) served as controls. The percentages of progressively motile (A) and live (B) cells after 1, 2, 4, 6 and 18-hour incubations were determined. The results are expressed as mean±s.e.m., n=7. *P<0.01 when compared with sperm motility under control conditions.





Right arrow Return to article