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Fig. 5. Effect of CaM kinase inhibitors upon hFF-induced acrosome reaction. After selection using glass wool columns, motile sperm were resuspended in medium not supplemented with CaCl₂ in the presence or absence of 60 µM KN62 or KN93. 30 minutes later, aliquots were supplemented with CaCl₂ (final concentration: 2.5 mM). Aliquots incubated in media not supplemented with Ca²⁺ (–Ca²⁺) and in the absence of the inhibitors (+Ca²⁺) served as controls. After an 18-hour incubation period, the sperm were exposed to 10% human follicular fluid (hFF) or buffer for 45 minutes and the acrosomal status determined using FITC-Pisum sativum agglutinin. The results are expressed as mean±s.e.m., n=4. *P<0.001 compared to acrosomal status of cells with no hFF added under all conditions and cells with hFF but no Ca²⁺.

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