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Fig. 4. Distributions of patterns after transfection of wild-type and mutant GFAPs into SW13Vim^– cells. (A) 1 day after transfection. (B) 3 days after transfection. For each plasmid and time point, cells on three or four coverslips were counted and assigned to a category based on their appearance with standard fluorescence microscopy, using a 100x oil objective. The mean proportions of cells with each pattern type are shown, along with error bars representing one standard deviation. R239C, mutant; wt, wild type; wt + m, co-transfection of wild-type and mutant GFAP plasmids. The difference between the proportion of cells with an exclusively `diffuse' pattern 1 day after transfection of mutant GFAP alone (57.8%) versus after co-transfection of wild-type and mutant GFAP (14.4%) is significant with a P value of 2.6 x 10^–4 by Student's t test. After 3 days, the difference remains significant with a P value of 4.5 x 10^–4 by Student's t test.

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