JCS02737 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. MKLP1 and HsCYK4 localize at the cell equator in PRC1-depleted cells. (A) Localization of PRC1, a-tubulin and DNA in control HeLa cell. (B, C) Localization of MKLP1 (B) or HsCYK4 (C) in PRC1-depleted cells. Central spindle microtubules were not bundled in PRC1-depleted cells (a-tubulin). Both MKLP1 and HsCYK4 dispersed from the central spindle microtubules but not disappear from the cell equator (B,C; upper panels, arrows) and accumulated at the equatorial cortex with RhoA (B,C; lower panels, arrowheads). Bar, 10 mm

• Movie 1 -

  Movie 1. Effects of nocodazole applied at initiation of furrowing in A6 cells expressing b-tubulin-GFP. Although the cleavage furrow formed at the equatorial cortex, it regressed immediately. The furrow repeated ingression and regression changing its position and finally the cell did not complete cytokinesis. Time-lapse videos were taken at a frame every 10 seconds and video shows 5 frames/second. Left, GFP image. Right, phase-contrast image.

• Movie 2 -

  Movie 2. HeLa cells treated with ECT2 siRNA for 36 hours. ECT2-depletion affects initiation of furrowing. Time-lapse videos were taken at a frame every 5 minutes and video shows 5 frames/second.

• Movie 3 -

  Movie 3. HeLa cells treated with HsCYK4 siRNA for 36 hours. HsCYK4-depletion affects initiation of furrowing. Time-lapse videos were taken at a frame every 5 minutes and video shows 5 frames/second.

• Movie 4 -

  Movie 4. HeLa cells treated with MKLP1 siRNA for 48 hours. MKLP1-depletion affects initiation of furrowing. Time-lapse videos were taken at a frame every 5 minutes and video shows 5 frames/second.