QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 5. ECT2, HsCYK4 and MKLP1 are required for RhoA localization during cytokinesis. (A) Depletion of ECT2, HsCYK4 and MKLP1 was confirmed by immunoblotting with each antibody. Control HeLa cells treated with a scrambled siRNA (C) and cells depleted of each protein by RNAi (RNAi). GAPDH was detected as a loading control. (B) RhoA disappears from the equatorial cortex during cytokinesis in cells depleted of ECT2 (ECT2 RNAi), HsCYK4 (HsCYK4 RNAi) or MKLP1 (MKLP1 RNAi). Cells were stained with antibodies to RhoA and -tubulin and DAPI. All images are X-Y images of cells projected to one plane from serial optical sections. (C) Quantification of RhoA accumulation in control and ECT2-, HsCYK4-, and MKLP1-depleted HeLa cells. RhoA accumulation is expressed as ratio of fluorescence intensity at the equatorial cortex over the fluorescence intensity at the polar cortex. Stars on the schematic drawing of cells (right) represent points for measuring RhoA intensity at the cell pole and the equator. In ECT2 (light grey bar; n=35), HsCYK4 (dark grey bar; n=36) and MKLP1 (black bar; n=39) siRNA-treated cells, RhoA accumulation is significantly reduced at the equatorial cortex compared with that in cells treated with scrambled siRNA (control; white bar; n=20). Bar, 15 µm.

Return to article