spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 4. The rate of protein degradation was similar between the wild-type and the deglycosylated mutants of ß-subunits. (A) Hep G2 cells transfected with control vector (Mock), the wild type (WT) or one of the three deglycosylated and FLAG-tagged ß-subunit genes (N124Q, N240Q, N124/240Q) were labelled with [35S]methionine for 15 minutes, followed by incubation in unlabelled culture medium for the time periods indicated. Whole-cell lysates were extracted and examined by anti-FLAG IP and autoradiography. (B) Quantitative analysis of the autoradiograph. The co-transfected EYFP tagged {alpha}-tubulin was used as a control for transfection efficiency. The 35S signals of the ß-subunit were first normalized by the signals of the co-transfected EYFP-{alpha}-tubulin. The data recorded at 1, 3 or 6 hours were normalized against that recorded immediately after the washout of [35S]methionine label (0 hour).





Right arrow Return to article