|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 2. Expression and cellular localization of NP60. (A) Total cell extracts from RAW264.7 cells were resolved by SDS-PAGE, and western blotting was performed using anti-NP60 antibody. (B) Total RNA was extracted from different cell lines, and used for PCR amplification with the primers as described in the Materials and Methods. NP60 is a 483 bp fragment. Cytoplasmic and nuclear proteins were prepared from RAW264.7 cells (C) and NP60-transfected 293T cells (D), resolved by SDS-PAGE and western blotted with antibodies against NP60 and actin. (E) 293T cells transfected with NP60-myc/his were fixed after transfection and used for immunohistochemistry assay. Signal was detected using his antibody; DNA was stained using DAPI. Bar, 10 µm.
|
