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Fig. 5. Phosphorylation assay for p38 ${alpha}$ in vitro. (A) NP60 expressed in 293T cells was incubated with p38 ${alpha}$ in the presence of [ ${gamma}$ -³²P]ATP with or without SB203580. The reaction complex was resolved by SDS-PAGE, and visualized by autoradiography. (B) NP60 incubated with p38 ${alpha}$ and ATF₂ in the presence of [ ${gamma}$ -³²P]ATP.

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