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Fig. 6 . Effect of MAPKKs on the phosphorylation of p38
induced by NP60. (A) Flag-tagged wild-type p38 (wt) and myc-NP60 were co-transfected into 293T cells. SB203580 (2 µM) was added to the medium 4 hours after transfection as indicated. Phosphorylation of p38 was analyzed by western blotting with anti-phospho-p38 (left panel). Flag-tagged mutant p38 [p38(M)] and myc-NP60 were co-transfected into 293T cells. Phosphorylation of p38 was analyzed as above (right panel). (B) Flag-p38 and myc-NP60 were co-transfected into 293T cells together with MKK6b(A) or MKK4(A). Phosphorylation of p38 was analyzed as in A. (C) Flag-p38 and myc-NP60 were co-transfected into 293T cells together with MKK6b or MKK4. Phosphorylation of p38 was analyzed as in A.
