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Fig. 2. Time- and temperature-dependence study of ROS production and PI staining in cells bearing temperature-sensitive mutations in orp2 or cdc18. The indicated strains, in log-phase, were shifted to the indicated temperatures for the indicated numbers of hours. Then DCDHFDA was added, and incubation was continued at the same temperatures for an additional 80 minutes. Other conditions were as described in Fig. 1 and supplementary material Fig. S1.





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