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Fig. 3. Dissociation of Mash1 from nuclear periphery is specific to neural fate. (A) RT-PCR shows Mash1 is upregulated upon neural differentiation of MR-7 ES cells by PA6 stromal cell layer method. Oct4 and Sox1 controls are also shown. (B) Frequency (mean ± s.d.) of FISH signals scored as peripheral in MR-7 ES cells, PA6-differentiated MR-7 neural cells, ex vivo Sox1-positive neural cells, mesoderm-differentiated OS25 cells, ex vivo wild-type keratinocyte precursors and ex vivo wild-type T cells. Also shown are undifferentiated OS25 ES and neural cells from Fig. 2, for reference. Numbers in bars represent total FISH signals scored. (C) Representative 2D FISH results for each of these cell types. Mash1 locus is green. Nuclei are counterstained with DAPI (blue). Bar, 5 µm.





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