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Fig. 5. Chromatin organisation at Mash1. (A) Histone modifications of Mash1 and neighbouring genes in ES and neural cells. Bars show relative levels of real-time PCR products amplified from ES (pink) and NE (blue) genomic DNA following ChIP with antibodies to AcH3K9, pan-AcH4, Me₃H3K9, Me₃H3K20 and Me₃H3K27. Results are normalized to histone H3 ChIP PCR levels. (B) Mash1 positioning in ES cells lacking chromatin-silencing factors. Bar chart shows number of Mash1 FISH signals scored as peripheral in Eed^-/-, G9a^-/-, SuV39h1^-/-, Dnmt1^-/- and Dnmt3ab^-/- ES cells. Also shown are OS25 ES and neural cells from Fig. 2, for reference. Numbers in bars represent total FISH signals scored. (C) Representative 2D FISH results for each of these knockout ES cells. Mash1 locus, green. Nuclei counterstained with DAPI (blue). Bar, 5 µm.

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