JCS02722 Supplementary Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1. Time-lapse confocal recording showing Jurkat T cells transfected a vector encoding GFP-b2-chimaerin C1 domain (GFP-C1). PMA (100 ng/ml) was added after the first frame, and images captured every 10 seconds.

• Movie 2 -

  Movie 2. Time-lapse confocal recording showing Jurkat T cells transfected with a vector encoding GFP-b2-chimaerin. PMA (100 ng/ml) was added after the first frame, and images captured every 10 seconds.

• Movie 3 -

  Movie 3. Time-lapse confocal recording showing Jurkat T cells transfected with a vector encoding GFP-b2-chimaerin Q32A. PMA (100 ng/ml) was added after the first frame, and images captured every 10 seconds.

• Movie 4 -

  Movie 4. Time-lapse confocal recording showing Jurkat T cells transfected with a vector encoding GFP-b2-chimaerin. PMA (800 ng/ml) was added after the first frame and images captured every 10 seconds.

• Movie 5 -

  Movie 5. Time-lapse confocal recording showing Jurkat T cells transiently transfected with a vector encoding GFP-b2-chimaerin, plated onto VCAM-1-coated slides. CXCL12 (200 ng/ml) was added after the first frame. Images were captured every 10 seconds.

• Movie 6 -

  Movie 6. Time-lapse confocal recording showing Jurkat T cells expressing a GFP-b2-chimaerin C1 domain mutant (GFP-F215G). Cells were plated onto VCAM-1-coated slides and CXCL12 (200 ng/ml) was added after the first frame. Images were captured every 10 seconds.