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Fig. 1. ß2-chimaerin is expressed in lymphoid cells. (A) Expression of ß2-chimaerin mRNA was determined in Jurkat T cells (JK) by RT-PCR using specific oligonucleotides; {alpha}1, {alpha}2 and ß2-expressing plasmids were used as controls. (B) ß2-chimaerin protein expression was analyzed in total lysates of the indicated lymphoid cell lines by western blotting with anti-ß2-chimaerin antibody. Membranes were re-probed with anti-tubulin antibody as a protein loading control. H, HT; HD, HDLM2; J, Jurkat; K, Karpas; M, Molt-4; N, Namalwa; SU, SU-DHL1; T, Toledo. (C) Subcellular localization of GFP-ß2-chimaerin in Jurkat T cells was examined by confocal microscopy (green, left), the Golgi was stained with the BODIPY TR fluorescent marker ceramide (red, center); merged fluorescence is shown in the right panel. Bar, 3 µm.





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