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Fig. 3. GFP-ß2-chimaerin-expressing Jurkat T cells show reduced PMA-induced F-actin polymerization. Jurkat T cells transfected with (A) GFP-ß2-chimaerin or (B) a GFP-coupled dominant-negative (RacN17) Rac mutant were stimulated with PMA (800 ng/ml, 5 minutes) or unstimulated (-), fixed and processed to determine GFP-ß2-chimaerin (A) or GFP-RacN17 (B) localization (green) and actin polymerization by Rhodamine-phalloidin staining (red). Control cells not expressing GFP constructs are indicated with an asterisk. Phase-contrast micrographs show the cells analyzed (left). Images are representative of the field observed for each condition. (C) Quantification of F-actin polymerization of experiments described in A and B was determined by analyzing intensity of fluorescence of Rhodamine-phalloidin staining. Values are the mean ± s.d. fluorescence intensity (arbitrary units) corresponding to 20 cells for each condition. Bars, 5 µm.





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