spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 4. GFP-ß2-chimaerin-expressing Jurkat T cells show reduced CXCL12-dependent cell spreading to immobilized VCAM-1. Jurkat T cells were transiently transfected with vectors encoding GFP, GFP-ß2-chimaerin or GFP-ß2-chimaerin plus RacV12. After 24 hours, cells were suspended in HBSS and plated onto VCAM-1-coated slides. Slides were mounted on a 37°C plate and analyzed by confocal microscopy. CXCL12 (200 ng/ml) was added after the first frame and images captured every 10 seconds. Time-lapse confocal recording is shown in Movie 5 in supplementary material. Images are shown at the indicated times to illustrate cell spreading. Bar, 10 µm.





Right arrow Return to article