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Fig. 9. GFP-ß2-chimaerin expression enhances CXCL12-dependent migration of Jurkat T cells. (A) The chemotactic response of parental (control) and GFP-ß2-chimaerin cells towards CXCL12 (200 ng/ml) was determined in a 5-µm-pore chemotaxis chamber (see Materials and Methods). Data are expressed as a percentage of input cells, and are shown as the mean ± s.d. of at least three independent experiments. (B) Chemotactic responses of parental (control) and GFP-ß2-chimaerin-expressing cells towards CXCL12 at the concentrations indicated were determined at 1 hour post-stimulation. Data are expressed as in A, showing the mean ± s.d. of at least three independent experiments. (C) The chemotactic response of parental, GFP- and GFP-ß2-chimaerin-expressing cells towards CXCL12 (200 ng/ml) was determined 1 hour post-stimulation. Before seeding into the chamber, cells were incubated with the indicated concentrations of U73122 or EGTA (30 minutes) or with PTx (100 ng/ml overnight) at 37°C. Data are expressed as in (A), and are shown as the mean ± s.d. of at least three independent experiments.
