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Fig. 8. Co-immunoprecipitation of plasma membrane- and ER-resident Ca2+-signaling proteins in WT and PERK-/- cells. (A) Extracts from pancreatic acini of three WT and three PERK-/- mice were used to measure expression (input) of the indicated proteins and for immunoprecipitation of PMCA. The immunoprecipitates were analyzed for co-immunoprecipitation of PMCA, IP3R3 and SERCA2b. (B) Extracts from UBSM of three WT and three PERK-/- mice were used to measure expression of the indicated proteins and for immunoprecipitation of Cav1.2. The immunoprecipitates were analyzed for co-immunoprecipitation of Cav1.2 and RyR2.





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