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Fig. 1. Antibody specificity and expression of UCH-L1 in the ventricular zone at E13. UCH-L1 expression was detected using immunohistochemistry with anti-PGP9.5. UCH-L1 is not detected in the brain of gad mice at E13 (A,B) but is strongly expressed in heterozygous littermates (A,B). Confocal microscopic images of coronal sections of gad mice and heterozygous littermates were double stained with antibodies for the progenitor marker nestin and UCH-L1 (PGP9.5) (A) or for the early neuronal marker tubulin ß III (TuJ1) and UCH-L1 (B). Long radial fibers are indicated by arrowheads, and various phases of progenitor cells are indicated by arrows (A). TuJ1-positive, migrating neuronal cells are indicated by arrowheads (B). MZ, marginal zone; VZ, ventricular zone. Bars, 40 µm.





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