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Fig. 2. Change in UCH-L1 expression pattern in the developing mouse brain. Cryosections of the brain at E14 and E16 were double stained with UCH-L1 and the neural progenitor marker nestin (A) or early neuronal marker TuJ1 (B). Unlike with UCH-L1, staining patterns for TuJ1 and nestin do not change between E14 and E16. At E14, UCH-L1 expression is higher in the VZ than in the MZ. At E16, higher expression of UCH-L1 is reciprocally detected in the CP. By contrast, at both E14 and E16, nestin is highly expressed in the VZ, and TuJ1 expression is higher in the MZ/CP. Fluorescence intensities per field (1700 µm²) were measured in each layer of the E14 and E16 brain and are shown to the right. Bars, 80 µm. (C,D) Higher-magnification images from A,B of UCH-L1 expression in the E14 and E16 brain: UCH-L1 and nestin (C); UCH-L1 and TuJ1 (D). UCH-L1 and nestin are co-expressed in the VZ at E14 and E16. Nestin is expressed only in radial glial fibers (arrowheads) of the CP but not in neurons. UCH-L1 expression level is high. A representative cell with a high level of UCH-LI expression is indicated by a white arrow and one with low expression is indicated by a yellow arrow (C). An early neuronal marker, TuJ1, was expressed in both migrating (arrowheads) and mature neurons (D). CP, cortical plate; IZ, intermediate zone; MZ, marginal zone; SVZ, subventricular zone; VZ, ventricular zone. Bars, 40 µm.

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