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Fig. 5. Morphological identification of subpopulations of cultured NPCs at 6 and 12 hours after induction of differentiation. Differentiating NPCs were double stained with UCH-L1 and nestin. For the quantification depicted in A, differentiating NPCs stained with UCH-L1 and nestin were classified as long, short or round (see text). For the quantification depicted in B, differentiating NPCs were classified based on three kinds of cell morphology: unipolar, bipolar, or multipolar.





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